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Forensics

Microreader™ 21 ID System

Microreader™ 21 ID System

The Microreader™ 21 ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions. 


For supplement autosome, X-chromosome and Y chromosome testing kits, please see "Microreader™ 23sp ID System", "Microreader™ 19X ID System".

Introduction

The Microreader™ 21 ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


Key Features

Classical commercial kit which has been used for more than 10 years domestically and worldwide.
■ Allows direct amplification from these common sample types used in paternity testing without the need for extraction or purification, such as blood and buccal samples deposited on swab or paper substrates, as well as extracted DNA from various sample.
■ Superior discrimination ability, with cumulative non-father exclusion rate CPE>0.999999995.
■ Improved genotyping accuracy through superior primer design, expanded allelic ladder and virtual bins.

Superior discrimination performance

It conforms to international standards and the forensic scientific DNA database construction specifications, has a high individual recognition rate, and its cumulative non-father exclusion rate is CPE>0.999999995, which is suitable for kinship testing, DNA database construction, and other forensic analysis. 

High quality reproductivity

This kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
① Well-resolved peaks above the background noise level
② Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
③ Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Direct amplification & higher efficiency

Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples. 

Anti-pollution design

The kit has an anti-pollution design. When performing STR typing it can remove other PCR products that may be mixed in the amplification tube, avoid personnel contamination or non-specific amplification, and effectively reduce the impact of laboratory aerosol contamination. Increase the success rate of the experiment.

Kit related published paper

[1] Wu, Heming, et al. "Invasive molecular prenatal diagnosis of alpha and beta thalassemia among Hakka pregnant women." Medicine 97.52 (2018). (DOI: 10.1097/MD.0000000000013557)
[2] Yang, Yaran, et al. "Haplotypic polymorphisms and mutation rate estimates of 22 Y-chromosome STRs in the Northern Chinese Han father–son pairs." Scientific reports 8.1 (2018): 1-6. (DOI: 10.1038/s41598-018-25362-3)

Product Details

Microreader™ 21 ID System Product Details

For Use with (Application)

Sequencing

Unit size

100/200 Reactions

Label or Dye

5 Dye

Product type

PCR amplification Kit

Detection method

Capillary Electrophoresis

Shipping Condition

Dry ice

Stroge Condition

Store at –25 to –15°C upon receipt and at 2–8°C after initial use

Target Loci

D19S433, D5S818, D21S11, D18S51, D6S1043, AMEL, D3S1358, D13S317, D7S820, D16S539, CSF1PO, PENTA D, D2S441, VWA, D8S1179, TPOX, PENTA E, TH01, D12S391, D2S1338, FGA